Abstract
Amaç: Bu çalışmada, östrüs siklusu
(proöstrüs, östrüs, metöstrüs, diöstrüs) boyunca endometriyumda görülen humoral savunma sistemi
hücreleri dağılımının histokimyasal olarak belirlenmesi amaçlanmıştır.
Yöntem: Ağırlıkları 200-250g arasında değişen üç aylık 28 adet
Wistar Albino dişi sıçan
(kullanıldı) incelemeye alındı. Sıçanlar, östrüs siklus evrelerine göre her
grupta 7 adet sıçan olacak şekilde 4 gruba ayrıldı. Östrüsun dönemlerini belirlemek
için, vajinal smear yöntemi uygulandı.
Alınan sürüntü temiz bir lam üzerine
yayılarak metilen
mavisi ile boyandı. Bu bloklardan alınan 6 µ kalınlığındaki kesitler plazma hücrelerini belirlemek için metil green-pironin boyama
yöntemi uygulandı.
Bulgular: Plazma hücrelerinin en fazla sayıda proöstrüs grubundaki sıçanlarda
görüldü. Plazma hücre sayısının sırasıyla östrüs, metöstrus ve diöstrus
evrelerine doğru oldukça azaldığı belirlendi.
Sonuç: Endometriyumdaki
humoral savunma sistemi hücrelerinin, östrüs siklusunun farklı evrelerinde
değişik dağılımlar gösterdiği sonucuna varıldı. Östrüs siklusunun, hormonal
regülasyonun kontrolü altında birbirini takip eden farklı evreler içerdiği ve
evreler arasındaki immunolojik tepkilerdeki farklılığın yine bu hormonal
kontroltü altında ortaya çıktığı kanaatine varıldı.
References
- 1. Bonatz, G, Hansman L, Buchholz F, Mettler L, Radzun JH and Semm, K. Macrophage and lymphocyte subtypes in the endometrium during different phases of the ovarian cycle, Int. J. Gynecol. Obstet, 1992;37: 29 - 36.
- 2. Markovic Saljnikov D, Pavlovic M, Simic M. Morphometric investigations of plasmocytes and detection of immunoglobulins in the female rat genital tract during the oestrous cycle, Acta Veterinaria (Belgrad) 1997;47(2-3):107-114.
- 3. Hawk HW, Brinsfield TH, Turner GD, Whitmore GW and M. A. Norcross. Effect of ovarian status on induced acute inflammatory responses in cattle uteri, Am. J. Vet. Res. 1964;25: 362-366.
- 4. Canning, B. M. and Billington, D. W. Hormonal regulation of immunoglobulins and plasma cells in the mouse uterus, J. Endocrin 1983; 97, 419 - 424.
- 5. Arda MO, Minbay A, Aydm N, Akay Ö, Îzgür M, Diker KS. immunoloji, 1. Baskl, Medisan Yaymevi, Ankara 1994.
- 6. Head RJ and Gaede DS. la antigen expression in the rat uterus, Journal of Reproductive Immunology 1986;9:137-153.
- 7. Mallenby J, Dunyer J, Howkins C and Hithen C. Effect of Experimental limbic on the estrus cycle and reproductive succes in rat. Epilessiya 1991;34(2):220-227.
- 8. Bancroft, J. D., Cook, H. C. (1984) Manual of histological techniques, Churchill Livingstone, New York
- 9. Böck, P. (1989) Romeis Mikroskopische Tecknik, 17. Aufl., Urban und Schwarzenberg, Munchen, Wien, Baltimore, 325 — 332.
- 10. .SPSS for Windows, Release 6.1 Standart Version, 1994,USA.
- 11. Akgül, A. (1997) Tlbbi Ara$lrmalarda Îstatistiksel Analiz Teknikleri, SPSS Uygulamalarl, YÖK Matbaasl, Ankara
- 12. Rachman F, Casimiri V, Psychoyos A and Bernard O. immunoglobulins in the mouse uterus during the oestrous cycle, J. Reprod. Fert. 1983;69:17-21.
- 13. Shaikh, AA. Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy, Biology of Reproduction. 1971;5:297-307.
- 14. Parr BM and Parr LE. Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract, J. Reprod. Fert. 1985;74:361-370.
- 15. Hussein, A. M. (1979)The reproductive tract immune system of the female pig. Ph. D. Thesis,University of Bristol.
- 16. Whitmore, H. L. , and Archbald, L. F. Demonstration and guantitation of immunoglobulins in bovine serum, follicular fluid, and uterine and vaginal secretions with reference to bovine viral diarrhea and infectious bovine rhinotracheitis. Am. J. Vet. Res. 1977;38: 455-457.
- 17. Mitchell, G. , Liu, I. K. , Perryman, L. E. , Stabenfeldt, G. H. and Hughes, J. P. Preferential production and secretion of immunoglobulins by the eguine endometrium-a mucosal immune system. J. Reprod. Fertil. 1982;32:161-168.
- 18. Wira, C. R. and Sandoe, C. P. (1980) Hormonal regulation of immunoglobulins influence of estradiol on immunoglobulins A and G in the rat uterus, Endocrinology, 106, 1020-1026.
- 19. Wira, C. R. and Sandoe, C. P. Sex steroid hormone regulation of IgA and IgG in rat uterine secretions, Nature, Lond. 1977;268:534-535.
- 20. Whitmore HL, Archbald LF. Demonstration and quantitation of immunoglobulins in bovine serum, follicular fluid, and uterine and vaginal secretions with reference to bovine viral diarrhea and infectious bovine rhinotracheitis. Am J Vet Res. 1977 Apr;38(4):455-7
- 21. Mitchell CA, Brown S, Campbell JK, Munday AD, Speed CJ. Regulation of second messengers by the inositol polyphosphate 5-phosphatases. Biochem Soc Trans. 1996;24:994–1000.
Abstract
Purpose:
In this study, it was histochemically
aimed to determine the distribution of humoral defense system cells in the
endometrium during oestrous cycle (proöstrüs, östrüs, metöstrüs, diöstrüs).
Method:
In this study, 28 Wistar Albino
female rats (used), weighing 200-250 g and 3 months old, were examined. The
rats were divided into 4 groups according to the estrous cycle stages as 7 rats
at each group. Vaginal smear method was used to determine estrous periods. The
receiving material was spread on a clean lam and painted with methylene blue.
Methyl green-pyronin staining method was used to identify plasma cells of 6 μ
thickness sections from parafine blocks.
Results:
The largest number of plasma cells
were seen in the proestrous group.
Plasma cell numbers were
significantly reduced towards oestrous, metrostrus and diestrus stages,
respectively.
Conclusion:
Humoral defense system cells in the
endometrium were found to show different distributions at different stages of
the oestrous cycle. The oestrous cycle contains different stages under the control of hormonal
regulation. It was concluded that the differences in the immunological
responses between these stages also appeared under this hormonal control.
References
- 1. Bonatz, G, Hansman L, Buchholz F, Mettler L, Radzun JH and Semm, K. Macrophage and lymphocyte subtypes in the endometrium during different phases of the ovarian cycle, Int. J. Gynecol. Obstet, 1992;37: 29 - 36.
- 2. Markovic Saljnikov D, Pavlovic M, Simic M. Morphometric investigations of plasmocytes and detection of immunoglobulins in the female rat genital tract during the oestrous cycle, Acta Veterinaria (Belgrad) 1997;47(2-3):107-114.
- 3. Hawk HW, Brinsfield TH, Turner GD, Whitmore GW and M. A. Norcross. Effect of ovarian status on induced acute inflammatory responses in cattle uteri, Am. J. Vet. Res. 1964;25: 362-366.
- 4. Canning, B. M. and Billington, D. W. Hormonal regulation of immunoglobulins and plasma cells in the mouse uterus, J. Endocrin 1983; 97, 419 - 424.
- 5. Arda MO, Minbay A, Aydm N, Akay Ö, Îzgür M, Diker KS. immunoloji, 1. Baskl, Medisan Yaymevi, Ankara 1994.
- 6. Head RJ and Gaede DS. la antigen expression in the rat uterus, Journal of Reproductive Immunology 1986;9:137-153.
- 7. Mallenby J, Dunyer J, Howkins C and Hithen C. Effect of Experimental limbic on the estrus cycle and reproductive succes in rat. Epilessiya 1991;34(2):220-227.
- 8. Bancroft, J. D., Cook, H. C. (1984) Manual of histological techniques, Churchill Livingstone, New York
- 9. Böck, P. (1989) Romeis Mikroskopische Tecknik, 17. Aufl., Urban und Schwarzenberg, Munchen, Wien, Baltimore, 325 — 332.
- 10. .SPSS for Windows, Release 6.1 Standart Version, 1994,USA.
- 11. Akgül, A. (1997) Tlbbi Ara$lrmalarda Îstatistiksel Analiz Teknikleri, SPSS Uygulamalarl, YÖK Matbaasl, Ankara
- 12. Rachman F, Casimiri V, Psychoyos A and Bernard O. immunoglobulins in the mouse uterus during the oestrous cycle, J. Reprod. Fert. 1983;69:17-21.
- 13. Shaikh, AA. Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy, Biology of Reproduction. 1971;5:297-307.
- 14. Parr BM and Parr LE. Immunohistochemical localization of immunoglobulins A, G and M in the mouse female genital tract, J. Reprod. Fert. 1985;74:361-370.
- 15. Hussein, A. M. (1979)The reproductive tract immune system of the female pig. Ph. D. Thesis,University of Bristol.
- 16. Whitmore, H. L. , and Archbald, L. F. Demonstration and guantitation of immunoglobulins in bovine serum, follicular fluid, and uterine and vaginal secretions with reference to bovine viral diarrhea and infectious bovine rhinotracheitis. Am. J. Vet. Res. 1977;38: 455-457.
- 17. Mitchell, G. , Liu, I. K. , Perryman, L. E. , Stabenfeldt, G. H. and Hughes, J. P. Preferential production and secretion of immunoglobulins by the eguine endometrium-a mucosal immune system. J. Reprod. Fertil. 1982;32:161-168.
- 18. Wira, C. R. and Sandoe, C. P. (1980) Hormonal regulation of immunoglobulins influence of estradiol on immunoglobulins A and G in the rat uterus, Endocrinology, 106, 1020-1026.
- 19. Wira, C. R. and Sandoe, C. P. Sex steroid hormone regulation of IgA and IgG in rat uterine secretions, Nature, Lond. 1977;268:534-535.
- 20. Whitmore HL, Archbald LF. Demonstration and quantitation of immunoglobulins in bovine serum, follicular fluid, and uterine and vaginal secretions with reference to bovine viral diarrhea and infectious bovine rhinotracheitis. Am J Vet Res. 1977 Apr;38(4):455-7
- 21. Mitchell CA, Brown S, Campbell JK, Munday AD, Speed CJ. Regulation of second messengers by the inositol polyphosphate 5-phosphatases. Biochem Soc Trans. 1996;24:994–1000.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Article |
| Authors | |
| Publication Date | April 15, 2016 |
| Submission Date | April 24, 2018 |
| Acceptance Date | May 9, 2018 |
| Published in Issue | Year 2016 Volume: 2 Issue: 1 |
